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KMID : 0895820230330040001
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Journal of Oriental Rehabilitation Medicine
2023 Volume.33 No. 4 p.1 ~ p.14
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Effects of Youngseonjeatonguem on Monosodium Iodoacetate-Induced Osteoarthritis in Sprague-Dawley Rats
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An Sung-Hu
Jeong Yeong-Jin
Kim Su-Hak
Lee Hark-Song
Yeom Seung-Ryong
Kwon Young-Dal
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Abstract
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Objectives : The anti-inflammatory and anti-arthritic effects of Youngseonjeatonguem (靈仙除痛飮, YSJTU) was evaluated in a cellular model using RAW264.7 macrophages, which are involved in osteoarthritis (OA), and an animal model using Sprague-Dawley (SD) rats, and a possible mechanism of anti-arthritic actions of YSJTU was presented.
Methods : RAW264.7 macrophages were activated by lipopolysaccharide (LPS). The production of pro-inflammatory cytokines (tumor necrosis factor [TNF]-α, interleukin [IL]-1β, and IL-6) and inflammatory mediators (nitric oxide [NO] and prostaglandin E2 [PGE2]) was determined by ELISA and Griess assay, respectively. Western blot was performed to determine inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) expression. OA was induced by intra-articular injection of monosodium iodoacetate (MIA) into the right knee joint of SD rats.
Results : In RAW264.7 macrophages, YSJTU reduced LPS-induced production of TNFα, IL-1β, and IL-6. In addition, YSJTU inhibited LPS-induced production of NO and PGE2 by suppressing iNOS and COX-2 expression. In SD rats, YSJTU improved MIA-induced OA by reducing swelling, skin heat, and cartilage degradation. In addition, YSJTU reduced serum levels of TNF-α, IL-1β, and IL-6, along with its significant decrease in serum levels of NO and PGE2.
Conclusions : These results suggest that YSJTU may exert anti-arthritic effect, at least in part, by inhibiting macrophage-mediated joint inflammation.
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KEYWORD
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Youngseonjeatonguem (Ling xi?n chu tong y?n), Osteoarthritis, Macrophages, Arthritis, Pro-inflammatory cytokines, Nitric oxide
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